The 73 S ribosome of Neurospora crassa is the native mitochondrial ribosome.
نویسندگان
چکیده
The effect of the inclusion of EDTA and of heparin, in media used in the isolation of mitochondria, on the mitochindrial ribosome has been investigated. 1. Mitochondria isolated from Neurospora crassa in the presence of EDTA contain only a single type of monomeric ribosome, viz. 73 S. 2. Mitochondria isolated in the presence of Mg 2÷ contain both 79-S and 73-S monomeric ribosomes. The heterogeneity of the ribosomes was demonstrated by (a) ultracentrifugation on sucrose gradients, (b) electron microscopy, (c) immunoprecipitat ion with antibodies against mitochondrial 73-S and 79-S cytoplasmic ribosomes, (d) gel electrophoresis of high and low molecular weight RNAs. 3. Inclusion of heparin in all media used for the isolation of mitochondria and ribosomes resulted in (a) dissociation of 73-S mitochondrial ribosomes into 50-S and 37-S subunits; (b) stabilization of 79-S cytoplasmic ribosomes; (c) in the case of mitochondria isolated in the presence of Mg 2÷ containing both 73-S and 79-S ribosomes, heparin causes the selective dissociation of the 73 S monosome to yield ribosomes containing only a single monomeric ribosome type, viz. 79 S. 4. It is concluded that (a) the 79-S ribosomes present in mitochondria isolated in the presence of Mg 2÷ are contaminating cytoplasmic ribosomes, (b) the 73-S ribosomes are the real functional mitochondrial ribosomes of Neurospora crassa.
منابع مشابه
Membrane-associated Ribosomes in Mitochondria of Neurospora Crassa
The poky (mi-1) strain of Neurospora crassa is a and the mass ratio of large to small subunit RNA useful test organism in which to evaluate methods is -10 :1 (1) . Nonetheless, mitochondrial protein for isolating mitochondrial ribosomes actively synthesis can be observed in poky (2) and cytoengaged in protein synthesis . In poky mitochondria chrome oxidase which is in part synthesized by there ...
متن کاملDefective production of mitochondrial ribosomes in the poky mutant of Neurospora crassa.
A strain of Neurospora crassa containing a cytoplasmic mutation (poky) affecting mitochondrial function is shown to be deficient in small ribosomal subunits in the mitochondrion during the exponential growth phase. In the stationary growth phase, small subunits are more abundant and are present in mitochondrial ribosomal monomers. This change can be correlated with the return of mitochondrial c...
متن کاملMitochondrial ribosome assembly in Neurospora. Two-dimensional gel electrophoretic analysis of mitochondrial ribosomal proteins
Recent results with Neurospora crassa show that one protein (S-5, mol wt 52,000) associated with the mitochondrial (mit) small ribosomal subunit is translated within the mitochondria (Lambowitz et al. 1976. J. Mol. Biol. 107:223-253). In the present work, Neurospora mit ribosomal proteins were analyzed by two-dimensional gel electrophoresis using a modification of the gel system of Mets and Bog...
متن کاملMitochondrial ribosome assembly in Neurospora. Structural analysis of mature and partially assembled ribosomal subunits by equilibrium centrifugation in CsCl gradients
In Neurospora, one protein associated with the mitochondrial small ribosomal subunit (S-5, Mr 52,000) is synthesized intramitochondrially and is assumed to be encoded by mtDNA. When mitochondrial protein synthesis is inhibited, either by chloramphenicol or by mutation, cells accumulate incomplete mitochondrial small subunits (CAP-30S and INC-30S particles) that are deficient in S-5 and several ...
متن کاملThe Intracellular Site of Synthesis of Mitochondrial Ribosomal Proteins in Neurospora Crassa
The intracellular site of synthesis of mitochondrial ribosomal proteins (MRP) in Neurospora crassa has been investigated using three complementary approaches. (a) Mitochondrial protein synthesis in vitro: Tritium-labeled proteins made by isolated mitochondria were compared to (14)C-labeled marker MRP by cofractionation in a two-step procedure involving isoelectric focusing and polyacrylamide ge...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Biochimica et biophysica acta
دوره 478 3 شماره
صفحات -
تاریخ انتشار 1977